For a double strand DNA, one strand is given below:

The amount of energy required to split the double strand DNA into two single strands is _____ \( \text{kcal mol}^{-1} \).
[Given: Average energy per H-bond for A–T base pair = \( 1.0 \, \text{kcal mol}^{-1} \), G–C base pair = \( 1.5 \, \text{kcal mol}^{-1} \), and A–U base pair = \( 1.25 \, \text{kcal mol}^{-1} \). Ignore repulsion between the phosphate groups.]